Hoʻohana ka "New Coronary Virus Pneumonia Diagnosis and Treatment Program (Trial Ninth Edition)" i ka ʻike nucleic acid i ka waiwai Ct ≥35 ma ke ʻano he kumu nui no ka hoʻokuʻu ʻana i ka hoʻokele kaʻawale a i ʻole ka hoʻokuʻu ʻana.No laila, he aha ka mea i hōʻike ʻia e ka waiwai Ct o ka pahu ʻike waika nucleic?Hoʻohālikelike ʻia nā waiwai Ct o nā pahu like ʻole?ʻOi aku ka haʻahaʻa o ka waiwai Ct, ʻoi aku ka maikaʻi o ka hana o ka pahu?
ʻO ka waiwai Ct (Threshold Cycle, Ct) ka helu o nā pōʻaiapuni PCR ke ʻoi aku ka ikaika o ka hōʻailona fluorescence quantitative fluorescence manawa maoli ma mua o ka paepae i hoʻonohonoho ʻia.Lawe ʻia ka lei aliʻi hou ma ke ʻano he laʻana nā pahu ʻike waika nucleic.No ka hopena like o ka pahu hoʻokahi e ʻike i ʻelua mau mea hoʻomaʻamaʻa i loaʻa i ka maʻi virus, ʻo A a me B, ʻo ka nui o ka waiwai Ct e hōʻike ana, i kekahi ʻano, ka helu o nā kope o ka gene viral, ʻo ia hoʻi, ka viral load.ʻO ka haʻahaʻa o ka Ct o ka laʻana B, ʻoi aku ka kiʻekiʻe o ka hoʻouka viral.Hoʻohālikelike ka waiwai Ct i ka ukana viral a me kona maʻi maʻi.ʻAʻole hiki ke noʻonoʻo ʻia nā mea maikaʻi a me nā pōʻino o nā kit quantitative fluorescence manawa maoli e ka nui o ka waiwai Ct, no ka mea, ʻaʻole like nā koina Ct o nā pahu like ʻole.Pono e ana ʻia ka loiloi hana o ka pahu mai nā ʻano o ka sensitivity (palena ʻike liʻiliʻi), kikoʻī, pololei, kūpaʻa, a me ka noi diagnostic.ʻO kēlā me kēia mea hana reagent he ʻumikumamāwalu mau ʻano ʻoihana kaua, a aia kā lākou mau mana kilokilo i ka hoʻohana ʻana, no laila ʻaʻole wau e hele i nā kikoʻī ma aneʻi.
No nā mea hana / ʻōnaehana hoʻopaʻa ʻana i ka PCR (QPCR) i ka manawa maoli, ʻike ʻia ka loaʻa ʻana o nā waiwai Ct a me kā lākou hoʻohana nui ʻana i nā noi ʻike pathogen i kamaʻāina i nā mea a pau.Me ka hoʻomohala ʻana o ka ʻoihana a me ka holomua ʻana o ka ʻenehana, ua loaʻa nā mea hana QPCR a me nā reagents i nā manawa a me nā pilikia.Ua ʻimi ʻo Yiou Think Tank (2021 Research Report on China's Genetic Testing Industry: Technology) i ka nui o nā ʻoihana ma ka ʻoihana QPCR o koʻu ʻāina e kū nei i nā pilikia maʻamau e pili ana i ka ʻenehana.I ka pane ʻana i kēia mau pilikia, ua hoʻokaʻawale ʻia nā hoʻonā i ʻelua ʻano: ka hoʻomaikaʻi ʻana i ka ʻenehana a me ka lawe ʻana i nā ʻenehana hou.
ʻO ka POCT no ka QPCR molecular diagnostics kekahi o nā kuhikuhi hoʻomohala o ka ʻoihana, a ʻo ka hoʻomaikaʻi ʻana i ka hana he mea nui ia e wikiwiki ai i ka noi ʻana o ka ʻenehana.No nā lako POCT me ka helu hoʻokahi o ka QPCR, pehea e hoʻonui ai i ka waiwai Ct o ka ʻāpana kī o kahi pane hoʻokahi ma R&D?ʻO nā mea hana like, ka ʻōnaehana hoʻohālikelike like, a me ke ʻano hoʻohālikelike like, ʻoi aku ka nui o ka waiwai Ct o kahi hopena hoʻokahi, ʻoi aku ka haʻahaʻa o ka pono o ka enzyme i ka hopena.
Ma ke kaʻina hana o ka hoʻomaikaʻi ʻana i ka hopena o ka enzyme a me ka hoʻomaikaʻi ʻana i ka waiwai Ct, hiki ke nānā mua ʻia kahi 40 PCR cycle fluorescence quantitative amplification curve.Hoʻokaʻawale ʻia ka pihi hoʻonui i ke kiʻi ma lalo i ka pae fluorescence background, exponential amplification phase, linear phase, a me plateau phase.I mea e hoʻokō ai i kahi kāʻei fluorescence haʻahaʻa, e like me ka hiki ke hoʻokaʻawale iā ia mai ka waiwai fluorescence o ka hoʻonui ʻana i ka exponential mua, pono e hoʻāʻo mai ka mea lawe hoʻopau hopena, ka nānā mua ʻana i ka hoʻolālā, a me ka ʻōnaehana pale.ʻO ka manawa hoʻonui exponential ka hopena pololei loa o ka hana enzyme, a ʻo ia hoʻi ka hōʻoia o ka hoʻonohonoho pono ʻana o nā lako POCT, nā mea lawe a me nā reagents.I kēia manawa, pono e hoʻoponopono ʻia a hoʻāʻo ʻia ka hoʻoponopono ʻana i ka wela o nā mea hana, ka loaʻa ʻana o ka hōʻailona optical a me ka nānā ʻana, ka biocompatibility carrier, a me ka hana ʻōnaehana o nā reagents.ʻO ka hope, ua hoʻopau ʻia ka hoʻonui holoʻokoʻa, a pono e hōʻike ʻia nā hopena e kahi algorithm data koʻikoʻi e loaʻa ai kahi waiwai Ct kūpono.
ʻO kahi ʻanuʻu liʻiliʻi i ka hoʻonui ʻana i ka waiwai Ct ka helu ʻole o nā limahana R&D.I loko o ka hopohopo a me ka hauʻoli o ke ao a me ka pō, i kēlā me kēia manawa "ʻaʻole i ʻike ʻia nā mauna a me nā muliwai, ʻeleʻele nā willows a nani nā pua a aia kekahi kauhale", e hāʻawi iā mākou i ka wiwo ʻole a me ka makemake e neʻe i mua. hou.
Ka manawa hoʻouna: Sep-29-2022